Transcriptome Analysis of Pyrethroid-Resistant Chrysodeixis includens (Lepidoptera: Noctuidae) Reveals Overexpression of Metabolic Detoxification Genes.
Identifieur interne : 000029 ( Main/Exploration ); précédent : 000028; suivant : 000030Transcriptome Analysis of Pyrethroid-Resistant Chrysodeixis includens (Lepidoptera: Noctuidae) Reveals Overexpression of Metabolic Detoxification Genes.
Auteurs : Clerison R. Perini [Brésil] ; Christine A. Tabuloc [États-Unis] ; Joanna C. Chiu [États-Unis] ; Frank G. Zalom [États-Unis] ; Regis F. Stacke [Brésil] ; Oderlei Bernardi [Brésil] ; David R. Nelson [États-Unis] ; Jerson C. Guedes [Brésil]Source :
- Journal of economic entomology [ 1938-291X ] ; 2020.
Abstract
Chrysodeixis includens (Walker, [1858]) is one of the most important defoliator of soybean in Brazil because of its extensive geographical distribution and high tolerance to insecticides compared with other species of caterpillars. Because of this, we conducted bioassays to evaluate the efficacy of pyrethroid λ-cyhalothrin on a C. includens resistant strain (MS) and a susceptible (LAB) laboratory strain. High throughput RNA sequencing (RNA-seq) of larval head and body tissues were performed to identify potential molecular mechanisms underlying pyrethroid resistance. Insecticide bioassays showed that MS larvae exhibit 28.9-fold resistance to pyrethroid λ-cyhalothrin relative to LAB larvae. RNA-seq identified evidence of metabolic resistance in the head and body tissues: 15 cytochrome P450 transcripts of Cyp6, Cyp9, Cyp4, Cyp304, Cyp307, Cyp337, Cyp321 families, 7 glutathione-S-transferase (Gst) genes, 7 α-esterase genes from intracellular and secreted catalytic classes, and 8 UDP-glucuronosyltransferase (Ugt) were overexpressed in MS as compared with LAB larvae. We also identified overexpression of GPCR genes (CiGPCR64-like and CiGPCRMth2) in the head tissue. To validate RNA-seq results, we performed RT-qPCR to assay selected metabolic genes and confirmed their expression profiles. Specifically, CiCYP9a101v1, CiCYP6ae149, CiCYP6ae106v2, CiGSTe13, CiCOE47, and CiUGT33F21 exhibited significant overexpression in resistant MS larvae. In summary, our findings detailed potential mechanisms of metabolic detoxification underlying pyrethroid resistance in C. includens.
DOI: 10.1093/jee/toaa233
PubMed: 33137187
Affiliations:
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Perini</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul</wicri:regionArea><placeName><region type="state">Rio Grande do Sul</region></placeName></affiliation></author><author><name sortKey="Tabuloc, Christine A" sort="Tabuloc, Christine A" uniqKey="Tabuloc C" first="Christine A" last="Tabuloc">Christine A. Tabuloc</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea></affiliation></author><author><name sortKey="Chiu, Joanna C" sort="Chiu, Joanna C" uniqKey="Chiu J" first="Joanna C" last="Chiu">Joanna C. Chiu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea></affiliation></author><author><name sortKey="Zalom, Frank G" sort="Zalom, Frank G" uniqKey="Zalom F" first="Frank G" last="Zalom">Frank G. Zalom</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis</wicri:cityArea></affiliation></author><author><name sortKey="Stacke, Regis F" sort="Stacke, Regis F" uniqKey="Stacke R" first="Regis F" last="Stacke">Regis F. Stacke</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul</wicri:regionArea><placeName><region type="state">Rio Grande do Sul</region></placeName></affiliation></author><author><name sortKey="Bernardi, Oderlei" sort="Bernardi, Oderlei" uniqKey="Bernardi O" first="Oderlei" last="Bernardi">Oderlei Bernardi</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul</wicri:regionArea><placeName><region type="state">Rio Grande do Sul</region></placeName></affiliation></author><author><name sortKey="Nelson, David R" sort="Nelson, David R" uniqKey="Nelson D" first="David R" last="Nelson">David R. Nelson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Memphis, TN.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Memphis</wicri:cityArea></affiliation></author><author><name sortKey="Guedes, Jerson C" sort="Guedes, Jerson C" uniqKey="Guedes J" first="Jerson C" last="Guedes">Jerson C. Guedes</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul</wicri:regionArea><placeName><region type="state">Rio Grande do Sul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of economic entomology</title><idno type="eISSN">1938-291X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chrysodeixis includens (Walker, [1858]) is one of the most important defoliator of soybean in Brazil because of its extensive geographical distribution and high tolerance to insecticides compared with other species of caterpillars. Because of this, we conducted bioassays to evaluate the efficacy of pyrethroid λ-cyhalothrin on a C. includens resistant strain (MS) and a susceptible (LAB) laboratory strain. High throughput RNA sequencing (RNA-seq) of larval head and body tissues were performed to identify potential molecular mechanisms underlying pyrethroid resistance. Insecticide bioassays showed that MS larvae exhibit 28.9-fold resistance to pyrethroid λ-cyhalothrin relative to LAB larvae. RNA-seq identified evidence of metabolic resistance in the head and body tissues: 15 cytochrome P450 transcripts of Cyp6, Cyp9, Cyp4, Cyp304, Cyp307, Cyp337, Cyp321 families, 7 glutathione-S-transferase (Gst) genes, 7 α-esterase genes from intracellular and secreted catalytic classes, and 8 UDP-glucuronosyltransferase (Ugt) were overexpressed in MS as compared with LAB larvae. We also identified overexpression of GPCR genes (CiGPCR64-like and CiGPCRMth2) in the head tissue. To validate RNA-seq results, we performed RT-qPCR to assay selected metabolic genes and confirmed their expression profiles. Specifically, CiCYP9a101v1, CiCYP6ae149, CiCYP6ae106v2, CiGSTe13, CiCOE47, and CiUGT33F21 exhibited significant overexpression in resistant MS larvae. In summary, our findings detailed potential mechanisms of metabolic detoxification underlying pyrethroid resistance in C. includens.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33137187</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1938-291X</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>02</Day></PubDate></JournalIssue><Title>Journal of economic entomology</Title><ISOAbbreviation>J Econ Entomol</ISOAbbreviation></Journal><ArticleTitle>Transcriptome Analysis of Pyrethroid-Resistant Chrysodeixis includens (Lepidoptera: Noctuidae) Reveals Overexpression of Metabolic Detoxification Genes.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">toaa233</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/jee/toaa233</ELocationID><Abstract><AbstractText>Chrysodeixis includens (Walker, [1858]) is one of the most important defoliator of soybean in Brazil because of its extensive geographical distribution and high tolerance to insecticides compared with other species of caterpillars. Because of this, we conducted bioassays to evaluate the efficacy of pyrethroid λ-cyhalothrin on a C. includens resistant strain (MS) and a susceptible (LAB) laboratory strain. High throughput RNA sequencing (RNA-seq) of larval head and body tissues were performed to identify potential molecular mechanisms underlying pyrethroid resistance. Insecticide bioassays showed that MS larvae exhibit 28.9-fold resistance to pyrethroid λ-cyhalothrin relative to LAB larvae. RNA-seq identified evidence of metabolic resistance in the head and body tissues: 15 cytochrome P450 transcripts of Cyp6, Cyp9, Cyp4, Cyp304, Cyp307, Cyp337, Cyp321 families, 7 glutathione-S-transferase (Gst) genes, 7 α-esterase genes from intracellular and secreted catalytic classes, and 8 UDP-glucuronosyltransferase (Ugt) were overexpressed in MS as compared with LAB larvae. We also identified overexpression of GPCR genes (CiGPCR64-like and CiGPCRMth2) in the head tissue. To validate RNA-seq results, we performed RT-qPCR to assay selected metabolic genes and confirmed their expression profiles. Specifically, CiCYP9a101v1, CiCYP6ae149, CiCYP6ae106v2, CiGSTe13, CiCOE47, and CiUGT33F21 exhibited significant overexpression in resistant MS larvae. In summary, our findings detailed potential mechanisms of metabolic detoxification underlying pyrethroid resistance in C. includens.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Perini</LastName><ForeName>Clerison R</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tabuloc</LastName><ForeName>Christine A</ForeName><Initials>CA</Initials><AffiliationInfo><Affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chiu</LastName><ForeName>Joanna C</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zalom</LastName><ForeName>Frank G</ForeName><Initials>FG</Initials><AffiliationInfo><Affiliation>Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stacke</LastName><ForeName>Regis F</ForeName><Initials>RF</Initials><AffiliationInfo><Affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bernardi</LastName><ForeName>Oderlei</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nelson</LastName><ForeName>David R</ForeName><Initials>DR</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Memphis, TN.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guedes</LastName><ForeName>Jerson C</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Econ Entomol</MedlineTA><NlmUniqueID>2985127R</NlmUniqueID><ISSNLinking>0022-0493</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">RNA-seq</Keyword><Keyword MajorTopicYN="N">chemical control</Keyword><Keyword MajorTopicYN="N">metabolic detoxification</Keyword><Keyword MajorTopicYN="N">soybean looper</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>2</Day><Hour>17</Hour><Minute>14</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33137187</ArticleId><ArticleId IdType="pii">5952278</ArticleId><ArticleId IdType="doi">10.1093/jee/toaa233</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li><li>États-Unis</li></country><region><li>Californie</li><li>Rio Grande do Sul</li><li>Tennessee</li></region></list><tree><country name="Brésil"><region name="Rio Grande do Sul"><name sortKey="Perini, Clerison R" sort="Perini, Clerison R" uniqKey="Perini C" first="Clerison R" last="Perini">Clerison R. Perini</name></region><name sortKey="Bernardi, Oderlei" sort="Bernardi, Oderlei" uniqKey="Bernardi O" first="Oderlei" last="Bernardi">Oderlei Bernardi</name><name sortKey="Guedes, Jerson C" sort="Guedes, Jerson C" uniqKey="Guedes J" first="Jerson C" last="Guedes">Jerson C. Guedes</name><name sortKey="Stacke, Regis F" sort="Stacke, Regis F" uniqKey="Stacke R" first="Regis F" last="Stacke">Regis F. Stacke</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Tabuloc, Christine A" sort="Tabuloc, Christine A" uniqKey="Tabuloc C" first="Christine A" last="Tabuloc">Christine A. Tabuloc</name></region><name sortKey="Chiu, Joanna C" sort="Chiu, Joanna C" uniqKey="Chiu J" first="Joanna C" last="Chiu">Joanna C. Chiu</name><name sortKey="Nelson, David R" sort="Nelson, David R" uniqKey="Nelson D" first="David R" last="Nelson">David R. Nelson</name><name sortKey="Zalom, Frank G" sort="Zalom, Frank G" uniqKey="Zalom F" first="Frank G" last="Zalom">Frank G. 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